The Effects of Amphiregulin Induced MMP-13 Production in Human Osteoarthritis Synovial Fibroblast
Figure 4
NF-κB is involved in the potentiation of MMP-13 production by AREG. (a) OASFs were pretreated for 30 min with PDTC (5 μM) and TPCK (5 μM) followed by stimulation with AREG for 24 h, and MMP-13 expression was examined by qPCR. (b) OASFs were pretreated for 30 min with PDTC (5 μM) and TPCK (5 μM) for 30 min followed by stimulation with AREG for 24 h; MMP-13 protein levels in cell lysates were determined by Western blot analysis. MMP-13 enzymatic activity in cell lysates and supernatants was measured using zymography. (c) OASFs were incubated with AREG for indicated time intervals; p-IKKα/β, p-IκBα, and p-p65 expression was determined by Western blot analysis. (d) OASFs were transfected for 24 h with IKKα and IKKβ mutant followed by stimulation with AREG for 24 h; and MMP-13 expression was examined by qPCR. Results are expressed as the mean ± SEM . compared with control; compared with AREG-treated group.
