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Mediators of Inflammation
Volume 2014 (2014), Article ID 759028, 12 pages
http://dx.doi.org/10.1155/2014/759028
Research Article

The Effects of Amphiregulin Induced MMP-13 Production in Human Osteoarthritis Synovial Fibroblast

1Department of Orthopedic Surgery, Shin Kong Wu Ho-Su Memorial Hospital, No. 95, Wen-chang Road, Shi-lin District, Taipei 11101, Taiwan
2Department of Orthopedic Surgery, National Taiwan University Hospital, No.1, Section 1, Ren-ai Road, Zhong-zheng District, Taipei 10051, Taiwan
3Central Laboratory, Shin-Kong Wu Ho-Su Memorial Hospital, No. 95, Wenchang Road, Shi-lin District, Taipei 11101, Taiwan

Received 30 April 2014; Revised 27 June 2014; Accepted 7 July 2014; Published 24 July 2014

Academic Editor: Luca Cantarini

Copyright © 2014 Yi-Te Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: EGF and TGF-alpha attenuate MMP-13 expression in OASFs. (a) OASFs were incubated with EGF (50 ng/ml) and TGF-alpha (50 ng/ml) for 24 h, and cell lysates were then collected. MMP-13 protein levels in cell lysates were determined by Western blot analysis. (n = 4). (b) OASFs were incubated with EGF (50 ng/ml) and TGF-alpha (50 ng/ml) for 24 h. The mRNA expression of MMP-13 was examined by qPCR (n = 4).

Figure S2: AREG slight induced AP-1 activation. OASF were incubated with various concentrations of AREG. AP-1 luciferase activity was measured, and the results were normalized to the β-galactosidase activity.

  1. Supplementary Figures