Impaired Resolution of Inflammation in the Endoglin Heterozygous Mouse Model of Chronic Colitis
Figure 5
Eng +/− mice show normal bone marrow-derived neutrophil function. (a) Superoxide () production, measured by a dihydroethidium (DHE) based assay, was tested in isolated bone marrow-derived neutrophils from days 0, 12, and 25, with or without PMA stimulation. The results are plotted as fold change relative to the unstimulated Eng +/+ control for each time point. versus corresponding unstimulated group. (b) Neutrophil migration capacity (displacement and velocity) was determined using fMLP as a chemotactic agent in both Eng +/+ and Eng +/− mice. Results represent mean ± SEM (for DHE assay: –8 samples for days 0, 12, and 25; for chemotaxis assay: experiments for each genotype).
