CD80 and CD86 Costimulatory Molecules Differentially Regulate OT-II CD4⁺ T Lymphocyte Proliferation and Cytokine Response in Cocultures with Antigen-Presenting Cells Derived from Pregnant and Pseudopregnant Mice

Figure 1

Proliferation and activation of OVA-specific T cells cocultured with splenic APCs isolated from mated or pseudopregnant female mice after blockade of CD80 and CD86. (a) Proliferation estimated by BrdU incorporation and ELISA. Data were presented as percentages of absorbance for blocked molecules in comparison with isotype control (mean ± SEM). (b) Activation of T cells was expressed as the ratio of total number of CD4⁺ CD25⁺ cells to the total number of CD4⁺CD25⁻ cells. Data were presented as percentages of CD4⁺CD25⁺/CD4⁺CD25⁻ for blocked molecules in comparison with isotype control (mean ± SEM). 𝑁=10 animals per group; Student’s 𝑡-test (parametric) or Wilcoxon (nonparametric) test, *𝑃<0.05; **𝑃<0.01.

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