Rapamycin Attenuates Endothelial Apoptosis Induced by Low Shear Stress via mTOR and Sestrin1 Related Redox Regulation

Figure 2

Rapamycin attenuated LSS induced ROS/RNS production in HUVECs. ROS/RNS accumulation caused by LSS at 2 dyne/cm² for 120 min was attenuated by rapamycin (RAPA) at the concentration of 100 ng/mL in HUVECs. (a) RAPA ameliorated the effect of LSS on promoting ROS production examined by mitoSOX. (b) Consistent result was observed with DHE staining. (c) RAPA attenuated LSS induced RNS accumulation assayed by DAF-2DA. Scale bars: 50 μm ((a)–(c)). (d) The line graph was the statistical results of fluorescence intensity and size at the fixed color threshold which showed the significant reduction of ROS or RNS production in RAPA + LSS group, *𝑃<0.05; 𝑛=10. (e) RAPA did not significantly increase ROS or RNS production as compared with vehicle DMSO, 𝑃=0.1279 with ANOVA, 𝑛=10.

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