Rapamycin Attenuates Endothelial Apoptosis Induced by Low Shear Stress via mTOR and Sestrin1 Related Redox Regulation

<table>Rapamycin attenuated LSS induced ROS/RNS production in RAECs. ROS/RNS accumulation caused by LSS at 2 dyne/cm<sup>2</sup> was attenuated by rapamycin (RAPA) at the concentration of 100 ng/mL in RAECs. (a) RAPA ameliorated the effect of LSS on promoting ROS production examined by mitoTracker. (b) Consistent result was observed with DHE staining. (c) Rapamycin attenuated LSS induced RNS accumulation assayed by DAF-2DA. Scale bars: 25 <i>μ</i>m ((a)–(c)). (d) The column graphs demonstrated the fluorescence intensity and size was significantly reduced in RAPA + LSS group as compared with LSS group, <svg height="11.375" id="M8" style="vertical-align:-0.30096pt" version="1.1" viewbox="0 0 58.712502 11.375" width="58.712502" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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Mediators of Inflammation

fig3

Figure 3

Figure 3: Rapamycin Attenuates Endothelial Apoptosis Induced by Low Shear Stress via mTOR and Sestrin1 Related Redox Regulation 