Rapamycin Attenuates Endothelial Apoptosis Induced by Low Shear Stress via mTOR and Sestrin1 Related Redox Regulation
Figure 4
LSS reduced sestrin1 expression and caused contradictory activation of mTORC1 and mTORC2 in HUVECs. LSS reduced sestrin1 expression and caused contradictory activation of mTORC1 and mTORC2 in HUVECs. (a) Gene expression of sestrin1 normalized to the house-keeping gene gapdh was significantly decreased after shearing HUVECs at 2 dyne/cm2 for 120 min compared to static cells,; . (b) Immunoblotting of mTORC1 activity at mTOR Ser2448 and S6K1 Thr389 phosphorylation sites (c). (d) The line graph below indicated the ratio of phosphorylated kinases to total proteins for each shearing time, with that of static cells set as 0 min. All data are from 3 independent experiments. (e) mTORC2 activity was determined by immunoblotting of Akt Ser473 phosphorylation with densimetric analysis of 3 independent experiments (f).
