Th9/IL-9 Profile in Human Echinococcosis: Their Involvement in Immune Response during Infection by Echinococcus granulosus

Figure 1

Quantitative RT-PCR. Total RNAs were extracted and subjected to reverse transcription, followed by determination by quantitative RT-PCR. The 2^−ΔΔCt method was used to determine the cycle number Ct value corresponding to a specific fluorescence threshold and quantify the target genes. β-Actin was used as an internal control. (a) The PU.1 and IL-9 mRNA expression in HC, CE, and PCE groups. (b) Relative expression values of PU.1 and IL-9 mRNA were calculated based on the values of β-actin. (c) The GATA-3 and IRF-4 mRNA expression in HC, CE, and PCE groups. (d) Relative expression values of GATA-3 and IRF-4 mRNA were calculated based on the values of β-actin. *𝑃<0.05; **𝑃<0.01, compared with the HC group. ^△𝑃<0.05; ^△△𝑃<0.01, compared with CE group. HC: healthy control; CE: CE pretherapy group; PCE: CE posttherapy group.

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