The Role of IL-17 Promotes Spinal Cord Neuroinflammation via Activation of the Transcription Factor STAT3 after Spinal Cord Injury in the Rat

Figure 2

(a) Representative IL-17 immunohistochemistry images in spinal cord tissue (brown granules, original magnification ×400). The sham-operated groups (A) and SCI group at 1 h (B), 24 h (C), 48 h (D), and 72 h (E). A digitized image shows labeled IL-17 immunohistochemistry in tissue. The gray value of the IL-17 immunohistochemical expression was assessed. IL-17 expression significantly increased in the rat SCI model group and peaked at 24 h. Bar equals 50 μm. (b) Morphometric quantitate of IL-17 protein expression in spinal cord tissues. The IOD in different groups was 0.19±0.03, 0.24±0.02, 0.40±0.02, 0.33±0.04, and 0.27±0.04, respectively. Representative spinal cord tissue sections from the sham-operated group (𝑛=15) and SCI groups at 1 h (𝑛=15), 24 h (𝑛=15), 48 h (𝑛=15), and 72 h (𝑛=15) rats. The data are presented as the mean ± the SE. ^∗𝑃<0.05 versus sham, ^#𝑃<0.05 versus 1 h, ^△𝑃<0.05 versus 24 h, and ^●𝑃<0.05 versus 48 h.

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