The Role of IL-17 Promotes Spinal Cord Neuroinflammation via Activation of the Transcription Factor STAT3 after Spinal Cord Injury in the Rat

Figure 3

(a) Representative p-STAT3 Immunohistochemical images in spinal cord tissue (brown granules, original magnification ×400). The sham-operated group (A) and SCI groups at 1 h (B), 24 h (C), 48 h (D), and 72 h (E). The digitized image shows labeled p-STAT3 immunohistochemistry on tissue. The IOD of p-STAT3 immunohistochemistry was assessed. The rat SCI model group showed significantly increased p-STAT3 expression and peaked at 24 h. Bar equals 50 μm. (b) Morphometric quantitate of p-STAT3 protein expression in spinal cord tissues. The IOD in different groups was 0.14±0.02, 0.26±0.02, 0.43±0.03, 0.35±0.02, and 0.30±0.03, respectively. Representative spinal cord tissue sections from the sham-operated group (𝑛=15) and SCI groups at 1 h (𝑛=15), 24 h (𝑛=15), 48 h (𝑛=15), and 72 h (𝑛=15) rats. The data are presented as the mean ± the SE. ^∗𝑃<0.05 versus sham, ^#𝑃<0.05 versus 1 h, ^△𝑃<0.05 versus 24 h, and ^●𝑃<0.05 versus 48 h.

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