Activation of Adenosine A3 Receptor Alleviates TNF-α-Induced Inflammation through Inhibition of the NF-κB Signaling Pathway in Human Colonic Epithelial Cells
Figure 5
Effect of 2-Cl-IB-MECA on TNF-α-induced IκB-α and p-IκB-α protein expression in HT-29 cells. HT29 cells were treated with various concentrations (10 nM, 30 nM, and 50 nM) of 2-Cl-IB-MECA for 30 min before TNF-α (10 ng/mL) stimulation for 30 min. Then total protein was extracted and analyzed by western blot. (a, c) TNF-α resulted in a reduction in IκB-α expression and an increase in p-IκB-α expression in HT-29 cells. (b, d) Compared with the TNF-α-only-treated group, 2-Cl-IB-MECA pretreatment enhanced the expression of IκB-α and attenuated the expression of p-IκB-α in a concentration-dependent manner. (e, f) Representative pictures of IκB-α protein expression in indicated groups detected by western blot. (g, h) Representative pictures of p-IκB-α protein expression in indicated groups detected by western blot. Data are shown as the mean ± SD from three independent experiments. * compared with the NC group; compared with the TNF-α-only-treated group; between the indicated groups.
