Development and Validation of Protein Microarray Technology for Simultaneous Inflammatory Mediator Detection in Human Sera

Figure 2

Microarray accuracy and precision of cytokine detection. PBS (a) and serum (b) spike experiments were performed for 16 cytokines at three known concentrations (22 pg/mL, 188 pg/mL, and 750 pg/mL). The expected (dashed lines) and observed concentration differences from these spiked experiments were determined by microarray. The accuracy of microarray cytokine detection was calculated for PBS (c) and serum (d) at each spike concentration. The majority of data fell within the acceptable limits of 80–120% accuracy, as outlined by the FDA [15]. The precision of microarray cytokine detection in PBS (e) and serum (f) spike experiments was also calculated. Again, the majority of data were within the limits of acceptability (<20% coefficient of variation, CV), as outlined by the FDA [15].

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