Development and Validation of Protein Microarray Technology for Simultaneous Inflammatory Mediator Detection in Human Sera

Figure 5

Bland Altman plots to compare ELISA and microarray. The acceptable level of bias is dictated by the thin dotted line on either side of zero. The dashed lines represent the 95% limits of agreement as the mean difference (2 SD). The differences between ELISA and microarray estimations show a notable advantage of the microarray technique in sensitivity especially for the low-abundance cytokines, such as IL-1β and IL-6.

(a) IL-6

(b) IL-4

(c) IL-8

(d) IL-1β

(e) TNF-α

(f) IL-10

(g) VEGF

(h) IL-17

(i) Eotaxin-1

(j) TGF-β

(k) MCP-1

(l) IFN-γ

(m) RAGE

(n) IL-23

(o) IP-10

(p) Eotaxin-2