Inhibition of Macrophage Functions by the C-Terminus of Murine S100A9 Is Dependent on B-1 Cells
Figure 4
Spreading and phagocytosis assays by B-1 cells after incubation with mS100A9p. (a) B-1 cells, isolated by stationary culture of peritoneal cells obtained from Swiss mice, were adhered per 3 h to coverslips (1 × 105 cells/well) and incubated with mS100A9p (0.59; 1.17; or 2.35 M in R-10, RPMI-1640 medium containing 10% of heat-inactivated fetal bovine serum, R-10), for 24 h at 37°C in 5% CO2. Control adherent cells were incubated only with R-10. (b) After spreading, cells were incubated with 1 × 106 Candida albicans particles for 1 h. Results are expressed as means ± SD, using six animals per group. ≤ 0.05, when compared to the control group (R-10).
