Metalloprotease Dependent Release of Placenta Derived Fractalkine
Figure 2
Analysis of fractalkine expression in human first trimester and term placenta tissue. Quantitative real-time RT-PCR analysis showed a 15.1-fold (±0.9) increase of fractalkine mRNA expression in term () compared to first trimester () placental tissues (a). Immunoblot analysis of first trimester and term placenta tissue homogenates detected a protein band, corresponding with recombinant 90 kDa human full length fractalkine (rhF), which was applied as positive control. Shown is one representative immunoblot (b). Semiquantitative band analysis revealed a 1.7-fold (±0.1) increase in fractalkine protein expression at term () compared to first trimester () (c). Values are given as mean ± SEM. .
