Metalloprotease Dependent Release of Placenta Derived Fractalkine

Figure 4

Effect of Batimastat on the release of soluble fractalkine in the trophoblast cell line BeWo. Increasing concentrations of the metalloprotease inhibitor Batimastat (5 μM and 10 μM, resp.) inhibited forskolin (20 μM) induced upregulation of fractalkine release in BeWo cells after 48 h (a). Inhibition of fractalkine release by Batimastat (10 μM) was accompanied with an increase in cell bound fractalkine in BeWo cells after 48 h (b). Batimastat (10 μM) slightly but not significantly decreased viability of BeWo cells compared to control (𝑃=0.456) (c). Data are presented as mean ± SEM from three independent experiments performed in triplicate. ^∗𝑃≤0.05, ^∗∗𝑃≤0.01, ^∗∗∗𝑃≤0.001.

(a)

(b)

(c)