Metalloprotease Dependent Release of Placenta Derived Fractalkine
Figure 5
Effect of Batimastat on the release of soluble fractalkine in placental explants. In first trimester placental explants Batimastat (10 μM) slightly but not significantly decreased the release of soluble fractalkine, whereas at the same time tissue associated fractalkine increased, compared to control (DMSO, 0.1%) after 5-day culture (a). Incubation of term placental explants with Batimastat (10 μM) led to a considerable decline in fractalkine release and was accompanied with an increase of the tissue associated fractalkine fraction after 5-day culture (b). Analysis of culture media from first trimester and term placental explants showed slight but not significant increase of released LDH activity in presence of Batimastat (10 μM) compared to control after 5-day culture (c). Data in (a) are presented as mean ± SEM from seven different explant experiments performed in triplicate. Data in (b) and (c) are presented as mean ± SEM from three different explant experiments performed in triplicate. , , .
