IVS1 −397T>C Estrogen Receptor α Polymorphism Is Associated with Low-Grade Systemic Inflammatory Response in Type 1 Diabetic Girls

Table 4

Flow cytometric analysis of CD4⁺IL17A⁺ T cells in girls differing in the IVS1 −397T>C estrogen receptor α polymorphism.

	CC	CT	TT	𝑃	𝑃^∗	𝑃^∗∗	𝑃^∗∗∗
CD4^+IL17A^+ T cells (%)
Control group	1.1 (0.9/1.4)	0.9 (0.7/1.3)	1.2 (0.8/1.5)	0.4	0.42	0.29	0.68
DM1	1.5 (1.1/2.1)	2.1 (1.5/3.25)	2.69 (2.04/3.7)	0.18	0.09	0.95	0.05

PBMCs from type 1 diabetic (DM1) and healthy (control group) girls were cultured and stimulated as described in Section 2 and then stained with antibodies against CD4 and IL17A. The percentage of CD4⁺IL17A⁺ T cells was determined by flow cytometry. Analyzing CD4⁺IL17A⁺ cells, dot plots representing anti-CD4 versus SS were carried out to establish CD4⁺ and CD4⁻ lymphocyte gates. Then, the anti-CD4 versus IL17A from CD4⁺ gate dot plot was generated and the frequency of Th17 cells was determined.
Results are shown as median and 10./90. percentile. Differences were calculated by the Kruskal-Wallis ANOVA test.
𝑃: the comparison between three analyzed genotypes CC, CT, and TT.
𝑃^∗: post hoc comparison between patients bearing CC and CT genotypes.
𝑃^∗∗: post hoc comparison between patients bearing CT and TT genotypes.
𝑃^∗∗∗: post hoc comparison between patients bearing CC and TT genotypes.