Angiogenin Reduces Immune Inflammation via Inhibition of TANK-Binding Kinase 1 Expression in Human Corneal Fibroblast Cells
Figure 2
Inflammatory cytokine profiles in HCF culture medium. (a) Treatment with TNF-α (20 ng/mL) resulted in amplification of five inflammatory cytokines and chemokines (GRO and -α, IL-6 and -8, and MCP-1). Treatment with ANG (2 μg/mL) resulted in reduction of these inflammatory cytokines and chemokines. Treatment with ANG alone only induced upregulation of ANF area. (b) Custom human growth factor antibody array map. Pos: positive control; Neg: negative control. (c) Relative density of inflammatory cytokines and chemokines. The signal intensities were observed and quantified using a chemiluminescence imaging device. The values presented in the bar graph are the mean ± SE from triplicate experiments ( versus control cells; versus TNF-α treated cells). GRO: growth regulated oncogene; MCP: monocyte chemotactic protein.
