Angiogenin Reduces Immune Inflammation via Inhibition of TANK-Binding Kinase 1 Expression in Human Corneal Fibroblast Cells
Figure 5
Western blot analyses of NF-κB in the nucleus and cytoplasm. (a) NF-κB nuclear translocation induced by TNF-α was diminished after ANG treatment. ANG treatment alone did not affect NF-κB nuclear translocation. (b) Densitometric analysis of the relative ratio of NF-κB in cells treated with TNF-α alone to those treated with TNF-α+ANG. The experiments were performed in triplicate (). (c) Immunocytochemistry of NF-κB in the nuclei and cytoplasm of HCFs. After treatment with ANG, the cells were fixed and then labeled with an anti-NF-κB antibody. Immunofluorescent images at higher magnification demonstrate attenuation of the expression of NF-κB in nucleus after treatment with ANG ((c) arrow) in cells treated with TNF-α (scale bar, 100 μm).
