Inflammation in the Disease: Mechanism and Therapies 2014View this Special Issue
Research Article | Open Access
Macadamia Oil Supplementation Attenuates Inflammation and Adipocyte Hypertrophy in Obese Mice
Excess of saturated fatty acids in the diet has been associated with obesity, leading to systemic disruption of insulin signaling, glucose intolerance, and inflammation. Macadamia oil administration has been shown to improve lipid profile in humans. We evaluated the effect of macadamia oil supplementation on insulin sensitivity, inflammation, lipid profile, and adipocyte size in high-fat diet (HF) induced obesity in mice. C57BL/6 male mice (8 weeks) were divided into four groups: (a) control diet (CD), (b) HF, (c) CD supplemented with macadamia oil by gavage at 2 g/Kg of body weight, three times per week, for 12 weeks (CD + MO), and (d) HF diet supplemented with macadamia oil (HF + MO). CD and HF mice were supplemented with water. HF mice showed hypercholesterolemia and decreased insulin sensitivity as also previously shown. HF induced inflammation in adipose tissue and peritoneal macrophages, as well as adipocyte hypertrophy. Macadamia oil supplementation attenuated hypertrophy of adipocytes and inflammation in the adipose tissue and macrophages.
The role of a diet with a higher content of unsaturated fatty acids, in place or concomitant to a diet with high content of lipids, has been appointed as an effective strategy to control metabolic disorders . Monounsaturated fatty acids (MUFA) rich diet has been reported to decrease plasma total cholesterol and LDL-cholesterol and increase HDL-cholesterol levels [2–5]. Moreover, when saturated fatty acids are replaced by MUFA in the diet of obese women, levels of inflammatory markers decrease, including IL-6 and visfatin in serum . Macadamia nut oil is rich in monounsaturated fatty acids, containing approximately 65% of oleic acid (C18:1) and 18% palmitoleic acid (C16:1) of the total content of fatty acids . Macadamia oil is the main source of palmitoleic acid in the human diet. Some studies have shown that diet rich in macadamia can improve the lipid profile [2, 8–10], but to date there is no studies on the effect of supplementation of macadamia oil on adipocyte hypertrophy and inflammation.
In 2008, Cao and colleagues  showed that mice deficient in lipid chaperones aP2 and mal1 present increased levels of palmitoleic acid in serum. Elevated levels of circulating palmitoleic acid restored sensitivity of insulin in liver and skeletal muscle, hepatosteatosis, and hyperglycemia, generated by high-fat diet. With this, the authors named this fatty acid as a lipokine, since palmitoleic acid has a hormonal-like effect .
The administration of high-fat diet in C57BL6 mice induces metabolic perturbations similar to those observed in humans. In fact, consumption of the high levels of saturated fatty acids is associated with overweight, visceral obesity, inflammation, dyslipidemia, and insulin resistance, in skeletal muscle, liver, and adipose tissue [12–17]. Saturated FFA promotes inflammation by interaction with toll-like receptor 4 (TLR4), activating NFB, JNK, and AP-1 pathways [18, 19].
A low grade inflammation is established with increase in plasma levels of IL-6, IL-1β, prostaglandins, TNF-α, and leptin and decrease in the production and secretion of adiponectin, IL-10, and IL-4 [20, 21]. The increase in local inflammation is potentiated by the recruitment of macrophages to adipose tissue and polarization of M2 macrophages (macrophages type 2) to M1 macrophages (macrophages type 1) [16, 22, 23].
The aim of our study was to evaluate the effect of macadamia oil supplementation, rich in MUFA (palmitoleic and oleic acids), on adipose tissue and peritoneal macrophages inflammation in mice fed a balanced diet or high-fat diet rich in saturated fatty acids. We measured glucose uptake (2-6 deoxyglucose uptake) and mRNA content of proteins (GLUT-4; IRS-1) involved in insulin signaling in soleus muscle. The contents of IL-10, IL-6, TNF-α, and IL-1β in peritoneal macrophages and adipose tissue were also determined. The adipocyte size was also evaluated.
2. Materials and Methods
All experiments were performed according to protocols approved by the Animal Care and Use Committee of the Institute of Biomedical Sciences, University of São Paulo. C57BL/6 male mice (8 weeks old) were used in this study. Animals were housed with light-dark cycle of 12-12 h and temperature of °C. Animals were divided into four groups: (a) control diet (CD), (b) high-fat diet (HFD), (c) control diet supplemented with macadamia nut oil (Vital Âtman, Uchoa, SP, Brazil) (CD + MO), and (d) high-fat diet supplemented with macadamia oil (HF + MO). Control groups were run concomitantly. The oil composition is shown in Table 1. During the first 4 weeks preceding the induction of obesity by HFD, all groups were ad libitum fed a control diet (76% carbohydrates, 9% fat, and 15% proteins). Similar protocol has been used in our previous studies [24, 25]. CD + MO and HF + MO were supplemented by oral gavage at 2 g per Kg of body weight, three times per week, during 12 weeks. This dosage of oil was chosen based on previous studies from our group using different oils with no signs of hepatic toxicity . CD and HF diet received water at the same dose.
|SFA = saturated fatty acids, sum of C12:0, C14:0, C16:0, C17:0, C18:0, C20:0, and C22:0; MUFA = monounsaturated fatty acids, sum of C16:1, C18:1n7, C18:1n9, C20:1n9, C20:1n11, and C22:1n9; PUFA = polyunsaturated fatty acids, sum of C16:3n4, C18:2n6, C18:3n3, and C22:5n3; PUFA n3 = sum of C18:3n3 and C22:5n3; PUFA n6 = C18:2n6.|
2.2. Serum Parameters Analysis
Serum triacylglycerol, total cholesterol, LDL-cholesterol, and HDL-cholesterol were determined by colorimetric assays (Labtest Diagnostics, Lagoa Santa, MG, Brazil). Serum glucose and insulin were measured using LABTEST colorimetric assay and radioimmunoassay (Millipore, Billerica, MA, USA), respectively, as described by Masi et al. (2012) . The HOMA index was determined by calculating fasting serum insulin (μU/mL) × fasting plasma glucose (mmol L−1)/22.5. Leptin and adiponectin were measured using the protocol of the manufacturing R&D system.
2.3. GTT and ITT
Glucose tolerance test (GTT) and insulin tolerance test (ITT) were carried out in all groups after 6 h fasting at the end of the 10th and 11th weeks of treatment, respectively.
The methodologies used for GTT and ITT were similar to that described by Masi et al. (2012) .
2.4. Insulin Responsiveness in Incubated Soleus Muscle
2.5. Haematoxylin and Eosin Staining
Adipose samples were fixed in formalin and paraffin embedded. Sections were prepared (5 μM) using Leica EG1150H Machine. Haematoxylin and Eosin (H&E) staining was conducted using Leica Autostainer XL and Leica CV5030. Sections were mounted using DPX media (Fisher Scientific, Ireland) and analyzed using Nikon 80i transmission light microscope.
2.6. Extraction of Fatty Acids from Gastrocnemius Muscle and Gas Chromatographic Analysis
Gastrocnemius muscle fragments (100 mg) were subjected to lipid extraction. For this, 0.5 mL chloroform/methanol (2 : 1; v/v) was added to 100 mg of gastrocnemius sample, well-vortexed and incubated at room temperature for 5 min. Additional volumes of 1.25 mL chloroform and 1.25 mL deionized H2O were then added, and finally, following vigorous homogenization for 3 min, samples were centrifuged at 1200 g for 5 min, at room temperature to obtain two phases: aqueous phase in the top and organic phase in the bottom containing. The organic phase was collected, dried, and suspended in isopropanol. Triglyceride content was then determined in the homogenate. After that, for fatty acid composition determination, gastrocnemius lipid extracts were dried using atmospheric N2 for evaporation of the solvent without fatty acid oxidation. The fractions of neutral and polar lipids were separated from these extracts by using a column chromatography. The polar (phospholipids) and neutral (triglycerides) fractions were methylated (for formation of methyl esters), using acetyl chloride and methanol. The methyl esters were analyzed in a gas chromatographer coupled to a flame ionizer detector (FID) (Varian GC 3900). Fatty acid composition was then determined by using standard mixtures of fatty acids with known retention times (Supelco, 37 Components).
For the analysis of fatty acids, a programmed chromatography was used with the characteristics described below. The reading was initiated at 170°C temperature for 1 minute and then a ramp of 2.5°C/min was employed to reach a final temperature of 220°C that was maintained for 5 min. The injector and detector were maintained at 250°C. We used the CP wax 52 CB column, with a 0.25 mm thickness, internal diameter of 0.25 mm, and 30 mm long, with hydrogen as the carrier gas.
2.7. Analysis of Inflammatory Parameters
2.7.1. Adipokines Content Measurements
Mice were euthanized on CO2 chamber and retroperitoneal adipose tissue was rapidly collected. About 100 mg of retroperitoneal adipose tissue was used for the determination of TNF-α, IL-6, and IL-10 content. Adipose tissue was homogenized in RIPA buffer (0.625% Nonidet P-40, 0.625% sodium deoxycholate, 6.25 mM sodium phosphate, and 1 mM ethylenediaminetetraacetic acid at pH 7.4), containing 10 g/mL of a protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA). Homogenates were centrifuged at 12.000 g for 10 min at 4°C, supernatant was collected, and protein concentration was determined using Bradford assay (Bio-Rad, Hercules, CA, USA). Bovine serum albumin was used as protein standard.
Ex Vivo Adipose Tissue Culture. Retroperitoneal adipose tissue explants (about 100 mg) were cultured in DMEM sterile medium (Gibco), containing 10% FBS, 2 mM glutamine, streptomycin, and penicillin for 24 h, at 37°C and 5% CO2, humidified air environment. Thereafter, medium culture was collected and used for the determination of IL-1β and IL-10, using ELISA assays (DuoSet kits, R&D System).
2.7.2. Peritoneal Macrophage Isolation and Culture
Cytokine and nitric oxide (NO) production were evaluated in macrophages obtained by washing the peritoneal cavity with 6 mL RPMI culture medium (Gibco), containing 10% FBS and 4 mM glutamine. Macrophage-rich cultures (more than 90% of the cells were F4/80+) were obtained by incubating peritoneal cells in 24-well polystyrene culture plates for 2 h at 37°C in a 5% CO2, humidified air environment. Nonadherent cells were removed by washing with RPMI. Adherent cells were then incubated with 2.5 μg/mL of LPS (E. coli, serotype 0111:B4, Sigma Chemical Company, USA) for 24 h . Medium was collected for determination of IL-6, IL-10, IL-1B and TNF-α by ELISA and nitrite content by Griess method .
2.8. Quantitative RT-PCR
Total RNA from the gastrocnemius muscle was extracted with Trizol reagent (Invitrogen Life Technologies, Grand Island, NY, USA), following the method described by Chomczynski and Sacchi . Reverse transcription to cDNA was performed using the high-capacity cDNA kit (Applied Biosystems, Foster, CA, USA). Gene expression was evaluated by real-time PCR , using Rotor Gene (Qiagen) and SYBR Green (Invitrogen Life Technologies) as fluorescent dye. Primer sequences are shown in Table 2. Quantification of gene expression was carried out using the RPL-19 gene as internal control, as previously described .
2.9. Statistical Analysis
Results are presented as mean ± S.D. All groups were compared by using two-way ANOVA followed by Bonferroni posttest. Significance level was set at .
3.1. Characterization of the Experimental Model
3.1.1. Body Composition
Mice fed high-fat diet showed increased body weight gain, hypercholesterolemia, and insulin resistance. These modifications were similar to those observed in our previous studies [24, 25]. Animals fed the high-fat diet (HF and HF + MO) for eight weeks showed increased (by 2-fold) body weight gain and visceral adiposity index as compared with CD and CD + MO (Table 3). The weights of the liver and the brown adipose tissue depot were not altered with diet or supplementation (Table 3). Although the visceral adiposity index of mice fed high-fat diet (HF and HF + MO) was greater than in animals that received control diet (CD and CD + MO), the HF group had an increase (by 1,62-fold) of adipocytes size compared to the control diet (Figures 1(a) and 1(b)), with statistical difference not evidenced in HF + MO group. No difference was evidenced by diet or supplementation in LDL-c, NEFA, and glycerol (data not shown). Moreover, the basal glycemia and were increased in both groups treated with high-fat diet (data not shown). Homa-IR index was increased in the HF group (by 3-fold) as compared to the other groups including the HF + MO (Figure 2(a)). This result suggests a beneficial effect of macadamia oil supplementation on insulin responsiveness in the HF group.
|Values represent the means ± S.D. of the data obtained from analysis of 15 animals per group. versus CD; versus CD + MO.|
The peripheral insulin resistance was confirmed by glucose uptake in incubated soleus muscle (data not shown), as also shown previously [24, 25]. In addition, both groups treated with high-fat diet showed decrease in GLUT-4 mRNA expression (Figure 2(b)). The PGC-1, IRS-1, CPT-1 and Perilipin 5 mRNA expression were not modulated in our treatment. The HF group showed an increase in triacylglycerol content in gastrocnemius muscle, but this effect was blunted in HF + MO (Figure 2(c)). The fatty acid composition in neutral or polar lipid fractions remains unchanged regardless of the diet given and MO supplementation (see Table 1 in Supplementary Material available online at http://dx.doi.org/10.1155/2014/870634).
3.2. Macadamia Oil Supplementation Attenuates High-Fat Diet Induced Inflammation
The contents of the anti-inflammatory cytokine IL-10 were increased in the HF + MO group (approximately 4,09-fold) (Figure 3(a)), while IL-1b concentration in the medium of adipose tissue explants was increased in the HF group (Figure 3(b)).
When stimulated with LPS, macrophages from all groups showed increased IL-6 production (by 2.97-fold) (Figure 4(d)), whereas IL-10 and NO production were elevated in cells from the HF and CT + MO groups (2.39- and 4.08-fold compared to base line, resp.) (Figures 4(a) and 4(e)). No effect of LPS stimulation was observed on TNF-α production by macrophages from all groups (Figure 4(c)).
Moreover, macrophages from the HF group showed an increase (by 2,41-fold) of IL-1β production compared to unstimulated cells whereas the supplementation with MO abolished this elevation (Figure 4(d)). Similar results were found in NO production. MO attenuated nitrate production by LPS stimulation on macrophages from the HF group (Figure 4(a)). The production of IL-10 was decreased in the CT group compared to CT + MO and HF groups (by 1,88-fold) (Figure 4(e)). TNF-α and IL-6 production remained unchanged by diet or supplementation (Figures 4(c) and 4(d)).
No significant difference was found in serum levels of adiponectin after 12 weeks of treatment (data not shown). As expected, leptin concentration was increased (by 5.69-fold) in the groups fed the high-fat diet (data not shown). The significant difference between the CD + MO and the CD + CT groups suggests that MO can enhance circulant leptin.
We showed herein that twelve weeks of macadamia oil supplementation attenuate the increase in inflammation and adipocyte hypertrophy in mice fed a high-fat diet that exhibit signs of the metabolic syndrome.
High consumption of fat, sucrose, and industrialized foods in association with sedentary lifestyle is the main contributor to obesity and its related comorbidities, including dyslipidemias, insulin resistance, and cardiovascular diseases; evidence has been accumulated that low grade inflammation plays a key role in the obesity induced comorbidities [11, 31–37].
The increase in adipocytes size was attenuated by macadamia oil treatment. The increase in adipocyte diameter has been associated with disturbances in cellular homeostasis, such as insulin resistance, inflammation, and hypoxia . The prevalence of large adipocytes increases leptin production and secretion, as observed in our study . The increase in leptin is associated with an elevation in low grade inflammation. Leptin is known to stimulate proinflammatory cytokines production in lymphocytes [40–42], monocytes , and macrophages .
Mice fed the HFD for 8 weeks exhibited increased IL-10 content in retroperitoneal adipose tissue. This result may be associated with the increase in peroxisome proliferator activated receptor- (PPAR-) gamma activity. This nuclear receptor increased the number of small adipocytes and raised the IL-10 [45, 46]. The increase of IL-10 content in adipose tissue leads to macrophage polarization (type 2) that is important for remodeling and tissue repair [47, 48]. Moreover, the increase in IL-10 content in adipocytes is associated with increased insulin sensitivity in adipose tissue [49, 50].
IL-1β strongly induces the inflammatory response in innate immune cells , via JNK and NFB pathway . IL-1β is also a potent inductor of insulin resistance. This cytokine decreased insulin-stimulated glucose uptake via ERK activation . Patients with high level of the circulating IL-1β are associated with greater risk on development of type 2 diabetes . Adipose tissue and peritoneal macrophages are two sources of IL-1β, and macadamia oil supplementation was effective in decreasing the production of this cytokine in both. However, unexpectedly, the CDM showed an increased IL-1β production after LPS stimulation in peritoneal macrophages.
NO production is increased in LPS-stimulated macrophages being more pronounced in mice fed high-fat diet . We demonstrated herein that the same pattern and the supplementation with macadamia oil prevented the production of NO by peritoneal macrophages from HF mice. Other bioactive compounds, such as epigallocatechin gallate and resveratrol , decrease NO production by macrophage inhibition through of MAP kinase, JNK, and NFB signaling .
In conclusion, macadamia oil supplementation attenuated inflammation and adipocyte hypertrophy in obese mice.
Conflict of Interests
The authors declare that they have no conflict of interests with the presented data.
The authors are grateful to Professor Rui Curi for the revision of the paper and for his constant support and encouragement. This work was supported by grants from Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ).
The fatty acid composition in neutral or polar lipid fractions was unchanged in gastrocnemius muscle, regardless of the diet and macadamia oil supplementation.
- C. Arapostathi, I. P. Tzanetakou, A. D. Kokkinos et al., “A diet rich in monounsaturated fatty acids improves the lipid profile of mice previously on a diet rich in saturated fatty acids,” Angiology, vol. 62, no. 8, pp. 636–640, 2011.
- J. Hiraoka-Yamamoto, K. Ikeda, H. Negishi et al., “Serum lipid effects of a monounsaturated (palmitoleic) fatty acid-rich diet based on macadamia nuts in healthy, young japanese women,” Clinical and Experimental Pharmacology and Physiology, vol. 31, supplement 2, pp. S37–S38, 2004.
- R. P. Mensink, P. L. Zock, A. D. M. Kester, and M. B. Katan, “Effects of dietary fatty acids and carbohydrates on the ratio of serum total to HDL cholesterol and on serum lipids and apolipoproteins: a meta-analysis of 60 controlled trials,” The American Journal of Clinical Nutrition, vol. 77, no. 5, pp. 1146–1155, 2003.
- T. A. Nicklas, J. S. Hampl, C. A. Taylor, V. J. Thompson, and W. C. Heird, “Monounsaturated fatty acid intake by children and adults: temporal trends and demographic differences,” Nutrition Reviews, vol. 62, no. 4, pp. 132–141, 2004.
- L. G. Gillingham, S. Harris-Janz, and P. J. H. Jones, “Dietary monounsaturated fatty acids are protective against metabolic syndrome and cardiovascular disease risk factors,” Lipids, vol. 46, no. 3, pp. 209–228, 2011.
- F. Haghighatdoost, M. J. Hosseinzadeh-Attar, A. Kabiri, M. Eshraghian, and A. Esmaillzadeh, “Effect of substituting saturated with monounsaturated fatty acids on serum visfatin levels and insulin resistance in overweight women: a randomized cross-over clinical trial,” International Journal of Food Sciences and Nutrition, vol. 63, no. 7, pp. 772–781, 2012.
- L. S. Maguire, S. M. O'Sullivan, K. Galvin, T. P. O'Connor, and N. M. O'Brien, “Fatty acid profile, tocopherol, squalene and phytosterol content of walnuts, almonds, peanuts, hazelnuts and the macadamia nut,” International Journal of Food Sciences and Nutrition, vol. 55, no. 3, pp. 171–178, 2004.
- A. E. Griel, Y. Cao, D. D. Bagshaw, A. M. Cifelli, B. Holub, and P. M. Kris-Etherton, “A Macadamia nut-rich diet reduces total and LDL-cholesterol in mildly hypercholesterolemic men and women,” Journal of Nutrition, vol. 138, no. 4, pp. 761–767, 2008.
- J. D. Curb, G. Wergowske, J. C. Dobbs, R. D. Abbott, and B. Huang, “Serum lipid effects of a high-monounsaturated fat diet based on macadamia nuts,” Archives of Internal Medicine, vol. 160, no. 8, pp. 1154–1158, 2000.
- N. R. Matthan, A. Dillard, J. L. Lecker, B. Ip, and A. H. Lichtenstein, “Effects of dietary palmitoleic acid on plasma lipoprotein profile and aortic cholesterol accumulation are similar to those of other unsaturated fatty acids in the f1b golden syrian hamster,” The Journal of Nutrition, vol. 139, no. 2, pp. 215–221, 2009.
- H. Cao, K. Gerhold, J. R. Mayers, M. M. Wiest, S. M. Watkins, and G. S. Hotamisligil, “Identification of a lipokine, a lipid hormone linking adipose tissue to systemic metabolism,” Cell, vol. 134, no. 6, pp. 933–944, 2008.
- M. D. Jensen, “Role of body fat distribution and the metabolic complications of obesity,” Journal of Clinical Endocrinology and Metabolism, vol. 93, no. 11, pp. S57–S63, 2008.
- C. L. Kien, “Dietary interventions for metabolic syndrome: role of modifying dietary fats,” Current Diabetes Reports, vol. 9, no. 1, pp. 43–50, 2009.
- V. T. Samuel, K. F. Petersen, and G. I. Shulman, “Lipid-induced insulin resistance: unravelling the mechanism,” The Lancet, vol. 375, no. 9733, pp. 2267–2277, 2010.
- D.-H. Han, P. A. Hansen, H. H. Host, and J. O. Holloszy, “Insulin resistance of muscle glucose transport in rats fed a high-fat diet: a reevaluation,” Diabetes, vol. 46, no. 11, pp. 1761–1767, 1997.
- M. F. Gregor and G. S. Hotamisligil, “Inflammatory mechanisms in obesity,” Annual Review of Immunology, vol. 29, pp. 415–445, 2011.
- H. H. Lim, S. O. Lee, S. Y. Kim, S. J. Yang, and Y. Lim, “Anti-inflammatory and antiobesity effects of mulberry leaf and fruit extract on high fat diet-induced obesity,” Experimental Biology and Medicine, vol. 238, no. 10, pp. 1160–1169, 2013.
- S. M. Reyna, S. Ghosh, P. Tantiwong et al., “Elevated toll-like receptor 4 expression and signaling in muscle from insulin-resistant subjects,” Diabetes, vol. 57, no. 10, pp. 2595–2602, 2008.
- J. Jin, X. Zhang, Z. Lu et al., “Acid sphingomyelinase plays a key role in palmitic acid-amplified inflammatory signaling triggered by lipopolysaccharide at low concentrations in macrophages,” American Journal of Physiology: Endocrinology and Metabolism, vol. 305, no. 7, pp. E853–E867, 2013.
- H. Waki and P. Tontonoz, “Endocrine functions of adipose tissue,” Annual Review of Pathology, vol. 2, pp. 31–56, 2007.
- N. Ouchi, J. L. Parker, J. J. Lugus, and K. Walsh, “Adipokines in inflammation and metabolic disease,” Nature Reviews Immunology, vol. 11, no. 2, pp. 85–97, 2011.
- S. P. Weisberg, D. McCann, M. Desai, M. Rosenbaum, R. L. Leibel, and A. W. Ferrante Jr., “Obesity is associated with macrophage accumulation in adipose tissue,” Journal of Clinical Investigation, vol. 112, no. 12, pp. 1796–1808, 2003.
- D. Patsouris, P.-P. Li, D. Thapar, J. Chapman, J. M. Olefsky, and J. G. Neels, “Ablation of CD11c-positive cells normalizes insulin sensitivity in obese insulin resistant animals,” Cell Metabolism, vol. 8, no. 4, pp. 301–309, 2008.
- L. N. Masi, A. R. Martins, J. C. R. Neto et al., “Sunflower oil supplementation has proinflammatory effects and does not reverse insulin resistance in obesity induced by high-fat diet in C57BL/6 mice,” Journal of Biomedicine and Biotechnology, vol. 2012, Article ID 945131, 9 pages, 2012.
- M. A. R. Vinolo, H. G. Rodrigues, W. T. Festuccia et al., “Tributyrin attenuates obesity-associated inflammation and insulin resistance in high-fat-fed mice,” The American Journal of Physiology—Endocrinology and Metabolism, vol. 303, no. 2, pp. E272–E282, 2012.
- J. M. Papadimitriou and I. Van Bruggen, “The effects of malnutrition on murine peritoneal macrophages,” Experimental and Molecular Pathology, vol. 49, no. 2, pp. 161–170, 1988.
- N. P. Sen and B. Donaldson, “Improved colorimetric method for determining nitrate and nitrate in foods,” Journal of the Association of Official Analytical Chemists, vol. 61, no. 6, pp. 1389–1394, 1978.
- P. Chomczynski and N. Sacchi, “Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,” Analytical Biochemistry, vol. 162, no. 1, pp. 156–159, 1987.
- R. Higuchi, G. Dollinger, P. S. Walsh, and R. Griffith, “Simultaneous amplification and detection of specific DNA sequences,” Bio/Technology, vol. 10, no. 4, pp. 413–417, 1992.
- W. Liu and D. A. Saint, “Validation of a quantitative method for real time PCR kinetics,” Biochemical and Biophysical Research Communications, vol. 294, no. 2, pp. 347–353, 2002.
- M. Matsuda and I. Shimomura, “Increased oxidative stress in obesity: Implications for metabolic syndrome, diabetes, hypertension, dyslipidemia, atherosclerosis, and cancer,” Obesity Research and Clinical Practice, vol. 7, no. 5, pp. e330–e341, 2013.
- C. Fernandes-Santos, R. E. Carneiro, L. de Souza Mendonca, M. B. Aguila, and C. A. Mandarim-de-Lacerda, “Pan-PPAR agonist beneficial effects in overweight mice fed a high-fat high-sucrose diet,” Nutrition, vol. 25, no. 7-8, pp. 818–827, 2009.
- J. C. Fraulob, R. Ogg-Diamantino, C. Fernandes-Santos, M. B. Aguila, and C. A. Mandarim-de-Lacerda, “A mouse model of metabolic syndrome: insulin resistance, fatty liver and Non-Alcoholic Fatty Pancreas Disease (NAFPD) in C57BL/6 mice fed a high fat diet,” Journal of Clinical Biochemistry and Nutrition, vol. 46, no. 3, pp. 212–223, 2010.
- G. D. Pimentel, A. P. S. Dornellas, J. C. Rosa et al., “High-fat diets rich in soy or fish oil distinctly alter hypothalamic insulin signaling in rats,” Journal of Nutritional Biochemistry, vol. 23, no. 7, pp. 822–828, 2012.
- L. Chen, D. J. Magliano, and P. Z. Zimmet, “The worldwide epidemiology of type 2 diabetes mellitus—present and future perspectives,” Nature Reviews Endocrinology, vol. 8, no. 4, pp. 228–236, 2012.
- C. J. Rebello, F. L. Greenway, and J. W. Finley, “A review of the nutritional value of legumes and their effects on obesity and its related co-morbidities,” Obesity Reviews, vol. 15, no. 5, pp. 392–407, 2014.
- K. E. Wellen and G. S. Hotamisligil, “Inflammation, stress, and diabetes,” Journal of Clinical Investigation, vol. 115, no. 5, pp. 1111–1119, 2005.
- S. L. Gray and A. J. Vidal-Puig, “Adipose tissue expandability in the maintenance of metabolic homeostasis,” Nutrition Reviews, vol. 65, supplement 1, pp. S7–S12, 2007.
- T. S. Higa, A. V. Spinola, M. H. Fonseca-Alaniz, and F. S. Anna Evangelista, “Comparison between cafeteria and high-fat diets in the induction of metabolic dysfunction in mice,” International Journal of Physiology, Pathophysiology and Pharmacology, vol. 6, no. 1, pp. 47–54, 2014.
- H. Kwon and J. E. Pessin, “Adipokines mediate inflammation and insulin resistance,” Frontiers in Endocrinology, vol. 4, article 71, 2013.
- G. M. Lord, G. Matarese, J. K. Howard, R. J. Baker, S. R. Bloom, and R. I. Lechler, “Leptin modulates the T-cell immune response and reverses starvation- induced immunosuppression,” Nature, vol. 394, no. 6696, pp. 897–901, 1998.
- P. Marzullo, A. Minocci, P. Giarda et al., “Lymphocytes and immunoglobulin patterns across the threshold of severe obesity,” Endocrine, vol. 45, no. 3, pp. 392–400, 2014.
- J. Santos-Alvarez, R. Goberna, and V. Sánchez-Margalet, “Human leptin stimulates proliferation and activation of human circulating monocytes,” Cellular Immunology, vol. 194, no. 1, pp. 6–11, 1999.
- S. C. Acedo, S. Gambero, F. G. P. Cunha, I. Lorand-Metze, and A. Gambero, “Participation of leptin in the determination of the macrophage phenotype: An additional role in adipocyte and macrophage crosstalk,” In Vitro Cellular and Developmental Biology - Animal, vol. 49, no. 6, pp. 473–478, 2013.
- A. Okuno, H. Tamemoto, K. Tobe et al., “Troglitazone increases the number of small adipocytes without the change of white adipose tissue mass in obese Zucker rats,” Journal of Clinical Investigation, vol. 101, no. 6, pp. 1354–1361, 1998.
- E. Rigamonti, G. Chinetti-Gbaguidi, and B. Staels, “Regulation of macrophage functions by PPAR- α, PPAR- γ, and LXRs in mice and men,” Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 28, no. 6, pp. 1050–1059, 2008.
- S. Fujisaka, I. Usui, A. Bukhari et al., “Regulatory mechanisms for adipose tissue M1 and M2 macrophages in diet-induced obese mice,” Diabetes, vol. 58, no. 11, pp. 2574–2582, 2009.
- A. Mantovani, A. Sica, S. Sozzani, P. Allavena, A. Vecchi, and M. Locati, “The chemokine system in diverse forms of macrophage activation and polarization,” Trends in Immunology, vol. 25, no. 12, pp. 677–686, 2004.
- E.-G. Hong, J. K. Hwi, Y.-R. Cho et al., “Interleukin-10 prevents diet-induced insulin resistance by attenuating macrophage and cytokine response in skeletal muscle,” Diabetes, vol. 58, no. 11, pp. 2525–2535, 2009.
- C. N. Lumeng, J. L. Bodzin, and A. R. Saltiel, “Obesity induces a phenotypic switch in adipose tissue macrophage polarization,” Journal of Clinical Investigation, vol. 117, no. 1, pp. 175–184, 2007.
- H. Tilg and A. R. Moschen, “Inflammatory mechanisms in the regulation of insulin resistance,” Molecular Medicine, vol. 14, no. 3-4, pp. 222–231, 2008.
- M. A. McArdle, O. M. Finucane, R. M. Connaughton, A. M. McMorrow, and H. M. Roche, “Mechanisms of obesity-induced inflammation and insulin resistance: insights into the emerging role of nutritional strategies,” Frontiers in Endocrinology, vol. 4, article 52, Article ID Article 52, 2013.
- J. Jager, T. Grémeaux, M. Cormont, Y. Le Marchand-Brustel, and J.-F. Tanti, “Interleukin-1β-induced insulin resistance in adipocytes through down-regulation of insulin receptor substrate-1 expression,” Endocrinology, vol. 148, no. 1, pp. 241–251, 2007.
- J. Spranger, A. Kroke, M. Möhlig et al., “Inflammatory cytokines and the risk to develop type 2 diabetes: results of the prospective population-based european prospective investigation into cancer and nutrition (epic)-potsdam study,” Diabetes, vol. 52, no. 3, pp. 812–817, 2003.
- Y. Zhong, Y.-S. Chiou, M.-H. Pan, and F. Shahidi, “Anti-inflammatory activity of lipophilic epigallocatechin gallate (EGCG) derivatives in LPS-stimulated murine macrophages,” Food Chemistry, vol. 134, no. 2, pp. 742–748, 2012.
- S. Han, J. H. Lee, C. Kim et al., “Capillarisin inhibits iNOS, COX-2 expression, and proinflammatory cytokines in LPS-induced RAW 264.7 macrophages via the suppression of ERK, JNK, and NF-κB activation,” Immunopharmacology and Immunotoxicology, vol. 35, no. 1, pp. 34–42, 2013.
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