Predictive Criteria to Study the Pathogenesis of Malaria-Associated ALI/ARDS in Mice
Figure 6
Increased vascular permeability and serum VEGF protein confirmed the predictive criteria for malaria-associated ALI/ARDS. (a) Serum VEGF protein in DBA/2 mice infected with P. berghei ANKA on the 7th DAI was measured by ELISA. The VEGF levels are higher in the mice classified as likely to die with ALI/ARDS compared with HP mice (according to the proposed predictive criteria). The data represent fold increases in relation to NI mice taken from three experiments; ( mice; , Mann-Whitney test). (b) Lung vascular permeability in DBA/2 mice infected with P. berghei ANKA 7th DAI, assessed using Evans Blue. The vascular permeability is higher in the mice classified as likely to die with ALI/ARDS compared with the HP mice (according to the proposed predictive criteria). The data represent fold increased in relation to NI mice taken from three experiments; ( mice; , Mann-Whitney test). Bars represent means and SEM. The images represent (c) NI: noninfected mice, (d) ALI/ARDS: acute lung injury acute respiratory distress syndrome, and (e) HP: hyperparasitemia.
