Research Article
Upregulating Nonneuronal Cholinergic Activity Decreases TNF Release from Lipopolysaccharide-Stimulated RAW264.7 Cells
Table 1
Sequences of primers used for real-time quantitative PCR analysis.
| | Gene symbol | Primer sequences | PCR product length (bp) |
| | TNF | F: ATGGGAAGGGAATGAATCCACC
R: GTCCACATCCTGTAGGGCGTCT | 281 |
| | ChAT | F: GTCTCTGAATACTGGCTGAATG
R: TGGTGTCTTGGAAGTGCTG | 106 |
| | AChE | F: ACTACCGAGTGGGAACCTTTGGC
R: CCTGTGGAAGAGGCTCCTGCTG | 224 |
| | -Actin | F: GCGTGACATCAAAGAGAAGC
R: AGCACTGTGTTGGCATAGAG | 270 |
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F: forward, R: reverse.
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