Uteroglobin, a Possible Ligand of the Lipoxin Receptor Inhibits Serum Amyloid A-Driven Inflammation
Figure 5
Attenuation of SAA induced cell migration and released IL-8 by hrUG in FLS. (a) Migration of FLS exposed to FBS, SAA (100 nM), or SAA with UG (100 nM), shown as % variation above control (basal growth medium). Results are the mean ± SD of three separate experiments with duplicate determinations. (b) Released IL-8 levels as measured by ELISA in the lower and upper chamber medium following FLS treatment with 5% FBS, SAA (100 nM) alone or in combination with UG or LXA4 (both at 100 nM). Results are the mean ± SD of duplicate determinations from two experiments. Data are normalized using cell numbers. (c) Migration of FLS following SAA (100 nM) treatment in lower chambers when IL-8 blocking antibody was added to lower, upper, or both compartments of the transwell system. Student’s -test SAA versus control values of <0.005 (**) and SAA versus SAA + UG treatments values of <0.01 () (a) and <0.002 () (b). Results are the mean ± SD from quadruple determinations of two experiments performed.
