An In Vitro Model to Evaluate the Impact of the Soluble Factors from the Colonic Mucosa of Collagenous Colitis Patients on T Cells: Enhanced Production of IL-17A and IL-10 from Peripheral CD4⁺ T Cells

Figure 3

Proliferation of anti-CD3 plus anti-CD28 stimulated CD4⁺ peripheral blood T cells () cultured in the absence or presence of two concentrations of conditioned medium (CM) derived from denuded biopsies (DNB) or from lamina propria mononuclear cells (LPMC) from inflamed CC mucosa or noninflamed controls, as analysed on day 3 of culture. Data are presented as percentage inhibition of proliferation compared to cells cultured in the absence of CM. Each symbol represents data from one blood donor and the data points from CC and noninflamed controls for each donor are connected with a line. For LPMC fraction, CD4⁺ peripheral blood T cells from four blood donors were tested for proliferation analysis.