Purine-Metabolizing Ectoenzymes Control IL-8 Production in Human Colon HT-29 Cells

Table 1

RT-PCR primers.

Gene	Forward primer	Reverse primer	Amplicon (bp)
P2RY1	AAAACTAGCCCCCTGCAACT	GATCTGATGCCGGATGAACT	153
P2RY2	CCACCTGCCTTCTCACTAGC	TGGGAAATCTCAAGGACTGG	163
P2RY4	GCAGGGATATCATGGGTGAC	CCCAGGAAGGAACAGAAACA	109
P2RY6	AGCTGGGCATGGAGTTAAGA	GCTGACTGGGACCTCTCAAG	139
P2RY11	CCTCTACGCCAGCTCCTATG	CCTCTACGCCAGCTCCTATG	211
P2RY12	TTTGCCCGAATTCCTTACAC	ATTGGGGCACTTCAGCATAC	192
P2RY13	CCCCTGGTACACTTGGAAGA	TACAGAGGAGGGGGTGATTG	125
P2RY14	TCTTTGGGCTCATCAGCTTT	TCCGTCCCAGTTCACTTTTC	213
ENTPD1	GCCAGCAGAAAAGGAGAATG	TGGGACCTTGGAATCACTTC	159
ENTPD2	TCAATCCAGCTCCTTGAACC	TCCCCAGTACAGACCCAGAC	167
ENTPD3	TTGACCTCAGGGCTCAGTTT	TGAGGGGGTTCACTGCTTAC	159
ENTPD8	ACTGGGCTACATGCTGAACC	GCACCATGAACACCACTTTG	107
GAPDH	CGACCACTTTGTCAAGCTCA	AGGGGTCTACATGGCAACTG	228
AK1β ^a	GGAATTCGACCATGGGCTGCTGCTC	GGAATTCGCAGCAGTGTGGGCTGTC	412
AK1β	ACAGGAGACACGGCAGGACGGGAC	CTCTTCTCCTTGCTGCACCTCC	385

aTaken from reference [46].