Protective Effects of Lipoxin A4 in Testis Injury following Testicular Torsion and Detorsion in Rats

Table 1

Apoptotic index, Johnsen scores, and caspase-3 activities in different groups (mean ± SD).

	Apoptotic index (%)	Johnsen scores	Caspase-3 activity; (absorbance 405/100 μg protein)
Sham	8.50±4.10^∗∗	7.50±0.80^∗	0.10±0.00^∗∗
Torsion	32.40±7.10	5.22±1.21	0.62±0.10
T/D	30.52±10.20	5.50±1.00	0.55±0.05
T/D + LXA4 (0.1 μg/kg)	24.60±7.70^#	5.80±1.00^#	0.42±0.12^##
T/D + LXA4 (1.0 μg/kg)	18.50±8.12^##	6.22±1.40^##	0.35±0.08^##
T/D + LXA4 (10 μg/kg)	14.30±6.75^##	7.15±1.50^##	0.22±0.05^##

T/D: torsion/detorsion; LXA4: lipoxin A4. Testicular injury and spermatogenesis were graded with Johnsen score. All tubular sections in each observed area of testicular tissue are evaluated systematically and each is given a score from 1 to 10. Complete spermatogenesis with many spermatozoa present is evaluated as score 10. Compared with other groups, ^∗𝑃<0.05 and ^∗∗𝑃<0.01. Compared with torsion or torsion/detorsion (T/D) group, ^#𝑃<0.05 and ^##𝑃<0.01.