Antagonizing Arachidonic Acid-Derived Eicosanoids Reduces Inflammatory Th17 and Th1 Cell-Mediated Inflammation and Colitis Severity
Table 3
Colonic mucosal mRNA expression in Wt and Fat-1 TNBS-treated mice1.
Gene
Wt
Fat-1
IL-17A
6.54 ± 2.95
2.04 ± 0.73*
IL-17F
6.14 ± 1.33
5.96 ± 1.03
IL-21
0.63 ± 0.09
0.15 ± 0.07*
IL-22
0.48 ± 0.17
0.95 ± 0.32
IL-23
1.63 ± 0.42
1.01 ± 0.41
IL-23R
1.72 ± 0.44
1.76 ± 0.28
IL-27
3.68 ± 0.84
7.50 ± 1.49*
CCL20
4.93 ± 1.59
4.25 ± 0.61
CCR6
0.45 ± 0.97
0.7 ± 0.48
ROR
15.79 ± 1.29
12.25 ± 0.82*
Tbet
2.30 ± 0.79
1.42 ± 0.46
Foxp3
1.74 ± 0.97
3.24 ± 0.63
IL-10
1.08 ± 0.68
2.64 ± 0.52*
TGF1
5.56 ± 0.66
6.91 ± 2.26
MCP-1
8.34 ± 3.75
3.03 ± 0.58*
IL-1
1.28 ± 0.87
0.39 ± 0.09*
TNF
1.90 ± 1.3
0.73 ± 0.18*
IL-6
2.88 ± 0.86
2.66 ± 0.61
IFN
0.63 ± 0.22
0.34 ± 0.15
IL-4
0.20 ± 0.11
0.28 ± 0.25
Mean values ± SEM of TNBS-treated Wt and Fat-1 mice ( = 8–10/genotype). All genes were measured within the same samples and expression was normalized to ribosomal 18S (in arbitrary units). For each gene, values marked with an asterisk differ from Wt ().