Antagonizing Arachidonic Acid-Derived Eicosanoids Reduces Inflammatory Th17 and Th1 Cell-Mediated Inflammation and Colitis Severity
Table 4
Colonic mucosal mRNA expression in Wt, Het, and Null Fads1 TNBS-treated mice1.
Gene
Wt
Het
Null
IL-17A
7.62 ± 4.70*
1.10 ± 0.32‡
0.31 ± 0.01‡
IL-17F
12.95 ± 3.71*
5.46 ± 1.07‡
4.19 ± 0.98‡
IL-21
1.53 ± 0.80
0.70 ± 0.18
0.47 ± 0.14
IL-22
1.48 ± 1.05
0.44 ± 0.33
0.10 ± 0.10
IL-23
3.88 ± 0.43*
3.58 ± 0.57*‡
2.19 ± 0.39‡
IL-23R
0.73 ± 0.16
0.40 ± 0.05
0.65 ± 0.21
IL-27
2.75 ± 0.67
1.86 ± 0.42
1.80 ± 0.46
CCL20
1.42 ± 0.49
1.36 ± 0.57
1.13 ± 0.30
CCR6
1.22 ± 0.38
0.82 ± 0.24
0.91 ± 0.44
ROR
11.07 ± 2.04*
8.75 ± 1.34*
5.28 ± 0.17‡
Tbet
8.35 ± 1.32*
3.30 ± 0.66‡
4.12 ± 0.69‡
Foxp3
2.95 ± 0.65
2.52 ± 0.23
1.81 ± 0.27
IL-10
13.57 ± 3.91*
9.08 ± 2.77*
3.95 ± 1.38‡
TGF1
1.80 ± 0.27
1.68 ± 0.26
1.31 ± 0.13
MCP-1
25.57 ± 8.37*
32.41 ± 14.00*
8.86 ± 3.49‡
IL-1
44.13 ± 12.57
31.30 ± 13.19
24.51 ± 12.95
TNF
9.85 ± 1.81
9.89 ± 3.82
5.65 ± 1.72
IL-6
11.54 ± 4.07*
7.11 ± 3.34*‡
2.10 ± 0.73‡
IFN
1.89 ± 1.01
0.52 ± 0.16
0.62 ± 0.21
IL-4
0.64 ± 0.36
nd
0.38 ± 0.73
Mean values ± SEM of TNBS-treated Wt, Het, and Null Fads1 mice ( = 6–9/genotype). All genes were measured within the same samples and expression was normalized to ribosomal 18S (in arbitrary units). For each gene, values not sharing a superscript symbol differ between genotypes (). nd, not detected.
