Glucagon-Like Peptide-1 Secreting Cell Function as well as Production of Inflammatory Reactive Oxygen Species Is Differently Regulated by Glycated Serum and High Levels of Glucose

Figure 1

GS and HG increase intracellular ROS without affecting GLUTag cell viability. After 5-day treatment in the presence of standard medium (CTR) or high glucose concentration (25 mmol/L) (HG) in presence of different concentrations of glycated serum (400 [GS] and 800 nmol/L [2GS]), ROS intracellular production (a) and cell viability (b) were assessed. Values shown indicate the percentage of absorbance compared to CTR. Data are expressed as the mean ± SE of 𝑛=5 (ROS release) or 𝑛=3 (cell viability) independent experiments. ^∗𝑃<0.05, ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 versus CTR.

(a)

(b)