Peritoneal Air Exposure Elicits an Intestinal Inflammation Resulting in Postoperative Ileus

Table 1

The primer sequences used for RT-PCR.

Target gene		PCR primer sequence	PCR product (bp)
GAPDH	Forward	5′-GGCATTGCTCTCAATGACAA-3′	223
	Reverse	5′-TGTGAGGGAGATGCTCAGTG-3′
TNF-𝛼	Forward	5′-ACTCCCAGAAAAGCAAGCAA-3′	211
	Reverse	5′-CGAGCAGGAATGAGAAGAGG-3′
IL-1𝛽	Forward	5′-AGGCTTCCTTGTGCAAGTGT-3′	230
	Reverse	5′-TGAGTGACACTGCCTTCCTG-3′
IL-6	Forward	5′-CTGCTCTGGTCTTCTGGAGT-3′	229
	Reverse	5′-GGTCTTGGTCCTTAGCCACT-3′
IL-10	Forward	5′-ATAACTGCACCCACTTCCCA-3′	231
	Reverse	5′-TTTCTGGGCCATGGTTCTCT-3′