IL-27 Activates Human Trophoblasts to Express IP-10 and IL-6: Implications in the Immunopathophysiology of Preeclampsia
Figure 5
The signaling molecule inhibitors affect the production of CXCL10 and IL-6 in HTR-8/SVneo. Analysis of the effects of AG, BAY, LY, SB, SP, or U on IL-27 induced CXCL10 and IL-6 release determined by enzyme-linked immunosorbent assay. HTR-8/SVneo cells were pretreated with AG490 (9 VM; AG), BAY1167082 (0.9 VM; BAY), LY294002 (8.4 VM; LY), SB203580 (36 VM; SB), SP600125 (7 VM; SP), or U0126 (20 VM; U) for 1 h followed by incubation for a further 24 h or 48 h with or without IL-27 (50 ng/mL). DMSO (0.1%) was used as the vehicle control. Results are expressed as the arithmetic mean ± SD from three independent experiments. * and *** when compared between groups denoted by horizontal lines.
