IL-27 Activates Human Trophoblasts to Express IP-10 and IL-6: Implications in the Immunopathophysiology of Preeclampsia

Table 2

Oligonucleotides used for PCR and real-time PCR.

Primer	Sequence
WSX-1:	5′-TGGACTTTTCCGAGGATGAC-3′; 3′-CTTAATGGACGACGACGAGG-5′
gp130:	5′-TGCTGATTGCAAAGCAAAAC-3′; 3′-CCTCACTTCTTCGTTCACCC-5′
IL-1𝛼:	5′-AGA AGAGACGGTTGAGTTTAAGCCAATCCA-3′; 3′-ATCCAG TCGTGGAAAATCGAAGGACTC-5′
IL-1𝛽:	5′-CAGGGACAGGATATGGAGCAACAA-3′; 3′-CATCTTTCAACACGCAGGACAGGT-5′
TNF-𝛼:	5′-AGGCCAAGCCCTGGTATG AGC-3′; 3′-CACAGGGCAATGATCCCAAAGTAG-5′
IL-6:	5′-CACCCCTGACCCAACCACAAAT-3′; 3′-TCCTTAAAGCTGCGCAGAATGAGA-5′
IL-10:	5′-CCGCCTCAGCCTCCCAAAGT-3′; 3′-CCCTAACCTCATTCCCCAACCAC-5′
IFN-𝛾:	5′-TAGCAACAAAAAGAAACGAGATGACT-3′; 3′-GATTTTGTCCCTTCGCTTTTTCC-5′
CXCL10:	5′-TGAATCAAACTGCGATTCTG-3′; 3′-GACTTTCGTCAATCGTTCCTTT-5′
GAPDH:	5′-CAG CGACACCCACTCCTC-3′; 3′-TGTCCCACCACCTGGAGT-5′