Hydrogen-Rich Saline Inhibits NLRP3 Inflammasome Activation and Attenuates Experimental Acute Pancreatitis in Mice

Figure 1

Evaluation of pancreatic NLRP3 inflammasome activation in mice by Western blot assay. The HRS at various doses or control saline (CS) was injected intraperitoneally to AP mice 1 h after the final injection of cerulein, respectively. Twenty-four hours later, animals in each group () were killed and the pancreatic tissue samples were immediately collected and then homogenized on ice. After the measurement of protein concentration, the NLRP3 inflammasome activation was evaluated by detecting the protein levels of TXNIP, NLRP3, and casp-1-p20 in pancreatic homogenates. L: low dose (2 mL/kg × 3); M: middle dose (4 mL/kg × 3); H: high dose (8 mL/kg × 3).