Mediators of Inflammation / 2014 / Article / Fig 6

Research Article

Leukocyte Production of Inflammatory Mediators Is Inhibited by the Antioxidants Phloretin, Silymarin, Hesperetin, and Resveratrol

Figure 6

Expression of TNF-α and IκB-α mRNA. PBMCs were cultured for 4 hrs in media alone, stimulated with Aa Y4 LPS (1 μg/mL), or treated with Silymarin or Resveratrol at a concentration of  M for 2 hrs prior to LPS challenge. PBMCs stimulated with LPS +/1 0.01% DMSO constituted the control. After 4 hrs, (a) TNF-α and (b) IκB-α mRNA expression levels were assayed by RT-PCR. Data is mean and SD of 3 technical replicates, 1 biological donor. Results were confirmed using a second biological donor. Student’s two-tailed -test was applied, indicating statistical significance versus LPS.

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