Protective Effect of Astaxanthin on Liver Fibrosis through Modulation of TGF-1 Expression and Autophagy
Figure 7
Effect of astaxanthin on HSC-T6, NCTC1469, and RAW264.7 cells. (a) The proliferation of HSC-T6 was detected by MTT. (b) The levels of -SMA, -pdfgr, and collagen I were detected by western blot. (c) The levels of beclin-1, LC3, TGF-1, Smad2, and Smad3 were detected by western blot. (d) The expression of -SMA in HSC-T6 cells was detected by immunofluorescence. (e) The apoptosis of NCTC1469 cells was detected by flow cytometry (, for TGF-1 + AST(40) versus TGF-1). (f) The result of western blot showed that astaxanthin decreased the expression of NF-B in RAW264.7 cells.
