Neutrophil Cathepsin G, but Not Elastase, Induces Aggregation of MCF-7 Mammary Carcinoma Cells by a Protease Activity-Dependent Cell-Oriented Mechanism
Figure 2
Effect of serine proteases on the adherence capacity of preadhered mammary carcinoma cells.(a) Induction of spheroidal aggregation by proteases against MCF-7 cells. (b) Induction of the loss of tight adhesion to culture substrates by serine proteases against MDA MB-231 cells. ((a), (b)) MCF-7 cells or MDA MB-231 cells were seeded in 96-well plates in RPMI 1640 medium containing 5% FBS. The cells were cultured overnight and the adherent cells were incubated with cathepsin G (), neutrophil elastase (●), chymotrypsin (), or trypsin () containing 1% BSA-medium for 24 h. After washing, residual cells were stained with crystal violet, and the aggregation index (a) or the detachment index (b) was calculated as described in Section 2. The results are expressed as mean (). When the bars are not shown, they are smaller than the size of the symbols. Lower photos: images of MCF-7 cells (left) and MDA MB-231 cells (right) at 24 h after incubation with the serine proteases. Original magnification: ×100.
