P2Y12 Receptor on the Verge of a Neuroinflammatory Breakdown
Figure 4
Temporal and regional pattern of P2Y12 expression in SOD1-G93A ALS spinal microglia. (a) Double immunofluorescence and confocal analysis on lumbar spinal cord sections (L3–L5) of wild-type (WT) mice was performed with c-ter antibody (green and yellow, merged and insets), CD11b (left panel, yellow, merged and inset), and CD68 (right panel, red, merged and inset), in both dorsal (DH) and ventral (VH) horns of spinal cord. (b) Double immunofluorescence and confocal analysis on SOD1-G93A lumbar spinal cord sections (L3–L5) at two different stages of ALS disease, that is, 20 weeks, and end stage, was performed with c-ter (green) and CD68 (red) antibodies. (c) Equal amount of total lumbar spinal cord lysates (L3–L5) from WT and SOD1-G93A ( for each group) were subjected to Western blotting and immunoreactions with c-ter and CD68 antibodies; anti-β-actin was used for protein normalization. Data represent means ± SEM. Statistical significance was calculated by Student’s -test, .
