IL-10 Counteracts Proinflammatory Mediator Evoked Oxidative Stress in Caco-2 Cells

Figure 1

Effect of 5-HT, adenosine, melatonin, TNFα, and/or IL-10 on lipid peroxidation (a) and protein oxidation (b). Caco-2 cells were treated throughout the course of one day with either 5-HT 100 μM, adenosine (Ado) 100 μM, melatonin (Mel) 5 mM, TNFα 5 ng/mL, or IL-10 at two concentrations, 0.01 or 25 ng/mL. Results were expressed as the percentages of the control value (100%) and were indicated as the mean±SE of five independent experiments. ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001, compared with control (C); ^#𝑃<0.05, ^##𝑃<0.01, and ^###𝑃<0.001 compared with the corresponding condition of treatment without IL-10.

(a)

(b)