BHBA Suppresses LPS-Induced Inflammation in BV-2 Cells by Inhibiting NF- κ B Activation

Figure 5

Effects of BHBA mediated by GPR109A. BV-2 cells were pretreated with vehicle or PTX for 1 h. Medium from each condition was removed and replaced with vehicle, BHBA (1.5 mM), PTX (100 ng/mL), LPS (1 μg/mL), BHBA + LPS, or BHBA + LPS + PTX. BV-2 cells were sampled at 4 h. The mRNA of proinflammatory enzymes and proinflammatory cytokines were determined by quantitative real-time PCR. Attenuation by BHBA of induced mRNA of iNOS (a), COX-2 (b), TNF-α (c), IL-1β (d), and IL-6 (e) from BV-2 cells; this effect is abolished with pretreatment with PTX (**𝑃<0.01).

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