BHBA Suppresses LPS-Induced Inflammation in BV-2 Cells by Inhibiting NF-
κ
B Activation
Figure 7
Effects of BHBA on LPS-induced phosphorylations of MAPKs. BV-2 cells were pretreated with or without BHBA (0.5, 1.0, and 1.5 mM) for 1 h and then were incubated with LPS (1 μg/mL) for 30 min. Cell lysates were prepared and subjected to western blotting by using pJNK54/46, pp38, or pERK1/2 antibodies, respectively. Each immunoreactive band was digitized and expressed as a ratio of β-actin levels. The ratio of the control group band was set to 1.00. Data are expressed as mean ± SD of three independent experiments. #Significantly different when compared with control alone, .
