Beetroot (Beta vulgaris L.) Extract Ameliorates Gentamicin-Induced Nephrotoxicity Associated Oxidative Stress, Inflammation, and Apoptosis in Rodent Model

Figure 4

Effect of BVEE on gentamicin-induced changes in inflammatory and apoptotic markers in kidney tissues of rats. (a) Immunoblots analysis of nuclear NF-κB (p65) expression in the kidney tissue of rats treated with BVEE and/or gentamicin. Β-Actin expression was used as a loading control. Representative bar diagram showing quantitative relative levels of NF-κB (p65) protein for vehicle, GM, and GM and BVEE treated rats. (b) Nuclear NF-κB (p65) DNA-binding activity determined by using NF-κB (p65) transcription factor ELISA assay kit. (c) Immunoblot analysis of apoptotic marker cleaved caspase-3 and Bax protein and antiapoptotic marker Bcl-2 protein in comparison with Β-actin expression was used as a loading control. The results are presented as the mean ± SEM of six animals per group. “*, **” *** denotes significant differences from the control group (, , and ); “^a,b,c” denote significant differences from the GM group (, , and ).

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