LPS from P. gingivalis and Hypoxia Increases Oxidative Stress in Periodontal Ligament Fibroblasts and Contributes to Periodontitis

Figure 2

Determination of ROS formation (H₂O₂) using Amplex Red assay. PDL cells were cultured under normoxic (Nox) or hypoxic condition (Hox) and stimulated with or without LPS of Porphyromonas gingivalis (1 μg/mL). H₂O₂ was analyzed after 1, 2 and 4 hours. Statistical differences were analyzed by one-way ANOVA and post hoc Dunnett and Tukey’s multiple comparison test; ^# difference to control; * difference between groups (means SD; ).