Figure 1: Effects of exercise protocols performed in different intensities on the TNF-α, IL-1 β, and NF-κB protein levels in the quadriceps of young and middle-aged rats. Protein levels of TNF-α (a), IL-1β (b), and NF-κB (c). Upper panels show representative blots of these proteins. Lower panels show representative blots of β-actin (a)–(c) protein levels. The results of scanning densitometry are expressed as arbitrary units. Bars represent means ± SEM of six rats. versus young NE rats, versus middle-aged NE rats, versus young rats at 0.8 km/h, and versus middle-aged rats at 0.8 km/h.