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Figure 5: Epithelial region of intestines harbored the cells exhibiting IL-17A mRNA expression increase during panobinostat treatment. In situ hybridization for cytokine mRNA was performed with RNAScope technology. (a–h) Plots depict the number of cell profiles producing mRNA for IL-17A (a and b), IFN-γ (c and d), IL-8 (e and f), and IL-1β (g and h) at baseline and during panobinostat. Left column depicts positive cell profiles per mm2 within the lamina propria regions. Right column depicts positive cell profiles per mm2 within the epithelial regions. Patient was excluded from the final analyses due to insufficient tissue for both optimization and assay performance. Each patient participant is represented in all figures by the same distinct symbol. Wilcoxon matched-pairs signed rank tests were used to generate the reported values.