Interplay between Intravitreal RvD1 and Local Endogenous Sirtuin-1 in the Protection from Endotoxin-Induced Uveitis in Rats

Figure 1

Resolvin 1 and sirtuin-1 in clinical development of EIU. Vehicle (saline + ethanol) and resolvin 1 (RvD1, 10–100–1000 ng/kg) were injected into the vitreous of rats 1 hour after vehicle + LPS (200 µg/rat) treatment. Clinical manifestations of EIU were evaluated 24 hours later both with hematoxylin and with eosin (representative pictures of RvD1 1000 ng; magnification: 100x and 200x, scale bar = 100 µm) and scored as reported in text (see Section 2). This figure also shows the effects of pretreatment with compound EX-527, an inhibitor of sirtuin-1 activity (10 mg/kg/day, i.p. for 7 days), prior to LPS and LPS + RvD1 (1000 ng/kg), and the effects of treatment with RvD1 FPR2/ALX Boc2 receptor antagonist (0.4 mg/kg/4 μL) into the vitreous (30 min prior to RvD1 1000 ng/kg). Values are reported as the mean ± S.E.M.; observations for each group. and versus LPS-treated rats; versus RvD1 1000 ng/kg.