4-Isopropyl-2,6-bis(1-phenylethyl)aniline 1, an Analogue of KTH-13 Isolated from Cordyceps bassiana, Inhibits the NF-κB-Mediated Inflammatory Response

Figure 3

Effect of KTH-13-AD1 on the activation of upstream signaling cascades of NF-κB. (a, b, c) Phospho- and total protein levels of IκBα, IKKα/β, AKT, p85, Src, Syk, and β-actin from cell lysates were determined through immunoblotting analysis. (d) The inhibitory activity of KTH-13-AD1 on the phosphorylation of p85 and Src, triggered by HA-Src overexpression in HEK293 cells, was evaluated using immunoblotting analysis. (e) NF-κB-mediated luciferase activity triggered by HA-Src overexpression in HEK293 cells, either in the presence or absence of KTH-13-AD1, was evaluated using luminometric analysis. compared to the vehicle control.

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