Histone Deacetylase Inhibitor Trichostatin A Ameliorated Endotoxin-Induced Neuroinflammation and Cognitive Dysfunction

Figure 6

TSA reduced the LPS-induced mRNA expression levels of HDACs and cytokines in BV-2 cells. One hour after BV-2 cells had been pretreated with saline or TSA (10 ng/mL), they were treated with saline or LPS for 4 hours as indicated. (a) HDAC2 and HDAC5 and (b) TNF-α, IL-1β, MCP-1, and IDO mRNA expression were determined using RT-PCR. The right panel shows the quantification of mRNA expression from three independent experiments. (c) The protein levels of TNF-α, IL-1β, and MCP-1 in supernatants of the cultured BV-2 cells were determined using ELISA. Data are means ± SEM ( in each group). compared with the Control group; ^# compared with the LPS-only group.

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