Inhibition of Epithelial CC-Family Chemokine Synthesis by the Synthetic Chalcone DMPF-1 via Disruption of NF-κB Nuclear Translocation and Suppression of Experimental Asthma in Mice

Figure 12

Effect of DMPF-1 on the mRNA level of chemokines and Th2 cytokines on OVA-sensitized mice. Expression levels of mRNA were determined by RT-PCR analysis. After the mice were sacrificed, the lung tissue was collected and the mRNA levels of (a) eotaxin-1, (b) RANTES, (c) IL-4, (d) IL-5, and (e) IL-13 were measured. The values are expressed as mean ± SEM (). , , and , significantly different from the OVA-challenged group.

(a)

(b)

(c)

(d)

(e)