High-Salt Enhances the Inflammatory Response by Retina Pigment Epithelium Cells following Lipopolysaccharide Stimulation

Figure 2

NaCl promotes the secretion of IL-6 and MCP-1 by ARPE-19 cells but had no effect on the production of IL-8. Cells precultured with serum-free medium for 24 h and then stimulated by LPS and cultured with medium alone or medium with an extra addition of NaCl (20 mM and 40 mM) for 24 h. The concentration of IL-6 ((a) ), MCP-1 ((b) ), and IL-8 ((c) ) in cell culture supernatants was measured by ELISA. and for comparison with control and NaCl-treated ARPE-19 cells. The data are expressed as mean ± SD of three independent experiments. Paired-samples t-test (when the difference between the two tested groups conforms to normal distribution) or Wilcoxon matched-pairs test (when the difference between the two tested groups does not conform to normal distribution) was used for statistical analyses for LPS control versus LPS + 20 mM NaCl or LPS + 40 mM NaCl in each group.

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